Genetic predictors of the effectiveness of the standard low-calorie diet in obese patients

Abstract


Objectives: The aim of the study was to investigate the genetic predictors of standard low-calorie diet at different polymorphic variations ApoE and LPL genes in obese patients.

Materials and methods: Our study involved 88 obese patients aged 19 to 67 years (27 men and 61 women, mean age 38.40 ± 2.82 years and the average BMI 41.71 ± 1.23). Study of lipid metabolism includes the determination of total cholesterol, triglycerides, low-density lipoprotein, high-density lipoprotein determined by spectrophotometry and immuno turbidimetry. Analysis of polymorphisms of LPL gene Ser447Ter (c.1595C>G), Cys112Arg (c.388T>C) and Arg158Cys (c.526C>T) of ApoE gene was performed by polymerase chain reaction.

Results: The results show significant differences in the efficacy of diet, depending on the genotype. We found that obese patients have significantly higher carrier genotypes c.388 T/T + c.526 C/C (by 20% reduced total cholesterol, by 19% – triglycerides, by20 % – low density lipoprotein, by 16% – high density lipoprotein, and by 9% – glucose), c.388 T/T + c.526 C/T ApoE gene (by 24% reduced total cholesterol, by 20% – triglycerides, and by 2% increased level of high-density lipoprotein) and C/C LPL gene (by 18% reduced total cholesterol concentration, by 17% – triglycerides, by 16% – high density lipoprotein, by 18% – low density lipoprotein, and by 9% – glucose).

Conclusions: Thus, ApoE and LPL genes alleles can be used as biomarkers of prognosis of efficiency of a standard low-calorie diet therapy in obese patients.


About the authors

Tatyana Sentsova

Federal State Institution of Science "Federal Research Centre of nutrition, biotechnology and food safety"; Moscow

Email: bio45@inbox.ru
SPIN-code: 7015-6690

Russian Federation MD, ScD, prof.

Olga Chernyak

Federal State Institution of Science "Federal Research Centre of nutrition, biotechnology and food safety"; Moscow

Author for correspondence.
Email: klinikakashir@yandex.ru
SPIN-code: 7496-8318

Russian Federation PhD

Ilya Vorozhko

Federal State Institution of Science "Federal Research Centre of nutrition, biotechnology and food safety"; Moscow

Email: bio45@inbox.ru
SPIN-code: 9117-5890

Russian Federation PhD

Kamila Gapparova

Federal State Institution of Science "Federal Research Centre of nutrition, biotechnology and food safety"; Moscow

Email: klinikakashir@yandex.ru

Russian Federation PhD

Olga Grigoryan

Federal State Institution of Science "Federal Research Centre of nutrition, biotechnology and food safety"; Moscow

Email: klinikakashir@yandex.ru
SPIN-code: 2713-6984

Russian Federation PhD

Yulya Chekhonina

Federal State Institution of Science "Federal Research Centre of nutrition, biotechnology and food safety"; Moscow

Email: klinikakashir@yandex.ru

Russian Federation postgraduate student

Alexandra Churycheva

Federal State Institution of Science "Federal Research Centre of nutrition, biotechnology and food safety"; Moscow

Email: klinikakashir@yandex.ru
SPIN-code: 3181-6767

Russian Federation PhD

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Copyright (c) 2016 Сенцова Т.Б., Черняк О.О., Ворожко И.В., Гаппарова К.М., Григорян О.Н., Чехонина Ю.Г., Чуричева А.М.

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